Regulating genome integrity

Understanding the interactions between biological macromolecules is crucial to permit dissection of  the array of complex processes within the cell. Detailed structural characterisation of complexes can enhance our knowledge of both these fundamental  processes and, equally, the diseases that ensue when they are disrupted. Such analysis can yield unanticipated insight and aid in the development of novel therapeutic interventions.

We were particularly interested in the structural analysis of protein-protein and protein-DNA interactions in the organisation of DNA replication and repair. Disruption of these core activities results in genome instability, with profound consequences for human health. The sliding clamp processivity factors (right) are central players in this regulation and form the core of our research.

In pursuit of this we employed a number of model systems and utilised innovative approaches in our research to enhance crystallisation of macromolecular complexes.